The answer
Potentially. Each test transaction creates outputs and consumes transaction fees; in some cases it adds an extra UTXO that later costs money to spend.
What to do next
For significant transfers, the verification benefit can outweigh the small cost. Use a sensible test amount and confirm addresses in full before the main payment.
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Frequently asked questions
Does a test transfer protect against address-replacement malware later?
Only partially. Re-check the address on the device for the actual large transfer.